Oxidative Stress and Antioxidant Status in Menstrual Cycle

Oxidative Stress and Antioxidant Status in Menstrual Cycle Evaluation of oxidative stress and antioxidant status during  normal menstrual cycle Abstract: Background Objectives: Oxidative stress has been investigated to explain various physiological as well as pathological basis of many medical conditions. But very few data concerning the oxidative stress during normal menstrual cycle of eumenorrheic women are available. Thus, the purpose of study was to examine the physiological role of oxidative stress during normal menstrual cycle. Methods: 120 young healthy female subjects of reproductive age group (17-27 yrs), having regular menstrual cycle, were examined. Serum malondialdehyde (MDA), an oxidative stress biomarker and serum ascorbic acid (vitamin-C), an antioxidant vitamin were assessed in the follicular phase (on 7th day) and in the luteal phase (on 21st day) of normal menstrual cycle. Results: In the present study, significant higher (p0.05) levels of ascorbic acid were observed in the luteal phase when compared to the follicular phase. Non-significant negative correlations were also observed between MDA and ascorbic acid in both the phases of normal menstrual cycle. Significant increase in serum MDA level coincided with the increased progesterone and estrogen levels during the luteal phase. High levels of estrogen may be the initiator of lipid peroxidation process which eventually ends up with cellular injury during the luteal phase. Interpretation Conclusion: Oxidative stress has an important role to play in physiological phenomenon of the menstruation. Keywords: Antioxidants, ascorbic acid, malondialdehyde (MDA), menstrual cycle, oxidative stress. Abbreviations: MDA, malondialdehyde. Introduction Menstrual cycle is the result of complex interacting processes involving interaction of the hypothalamus, pituitary, ovaries, uterus, prostaglandins and neuroendocrine factors1. The normal menstrual cycle is a twenty eight day period which is divided into two phases i.e. follicular phase and luteal phase. The follicular phase is characterised by a low level of estrogen and progesterone which is followed by rise in estradiol, lutenizing hormone, and follicle stimulating hormone at the time of ovulation, while the luteal phase is associated with increased levels of progesterone and estrogen. These phases are associated with various changes in blood parameters along with variations in the sex hormones. Several powerful reactive oxygen species or free radicals or oxidants are produced during the course of metabolism in blood cells and most other cells of the body. These oxidants are very reactive molecules that can react with proteins, nucleic acids, lipids and other molecules which changes physicochemical status of the cell to alter their structure and cause tissue damage. Lipid peroxidation is a well established mechanism of cellular injury in human and is used as an indicator of oxidative stress in cells and tissues. Malondialdehyde (MDA) is one of the important byproduct of lipid peroxidation process which is widely used as an indicator of lipid peroxidation2. These free radicals are the target for the enzymatic and non-enzymatic scavenging systems3, which includes the antioxidants such as superoxide dismutase, glutathione peroxidase, vitamin-A, vitamin-C, vitamin-E etc. that scavenge the free radicals and protect the tissues from oxidative stress. Oxidative stress has been implicated in various physiological as well as pathological bases of many medical conditions including reproductive system. Evidences have shown the dual effects of oxygen radicals in the physiological reproductive processes such as oocyte maturation, ovulation, menstruation, luteolysis, luteal maintenance in pregnancy, implantation and blastocyst development4,5,6, as well as in the pathological conditions like spontaneous abortions and infertility in females7. The imbalance between free radicals and antioxidants resulting from either an overproduction of free radicals or a deficit in antioxidant protection leads to oxidative stress8. Although, reports regarding variations of oxidative stress across the normal menstrual cycle in eumenorrheic women have been published, specially in reference to MDA (a marker of oxidative stress and an important byproduct of lipid peroxidation), but these are sparse and have conflicting trend. Thus, the purpose of this study was to examine the role of oxidative stress during the different phases of normal menstrual cycle by measuring the MDA which is served as an oxidative stress biomarker and ascorbic acid (vitamin-C), served as an antioxidant. Materials and Methods The present study was carried out on 120 normal healthy and regular menstruating female subjects aged between 17 and 27 years (mean age 20.53 + 2.9 years) selected from thexxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx. Study was carried out in the xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxPermission was also obtained from institutional ethical committee for carrying out the research work. After seeking an informed written consent and recording the detailed menstrual history (i.e. age at menarche, date of last menstruation, cycle length and days of bleeding), the subjects were then subjected to sample collection. Serum malondialdehyde (MDA) and serum ascorbic acid (vitamin-C) levels were assayed during their monthly menstrual cycle. All subjects were neither obese nor on any medication of any known pathologies. 5 ml of fasting blood samples were drawn from antecubital vein from each subject during the follicular phase (on 7th day of the cycle) and luteal phase (on 21st day of the cycle) after taking full antiseptic precautions. The MDA levels were measured by thiobarbituric acid assay method9. The principle of the method was based on the spectrophotometric measurement of the colour developed during the reaction of thiobarbituric acid with MDA. The concentration of thiobarbituric acid reactive substances was calculated by the absorbance coefficient of malondialdehyde-thiobarbituric acid complex. Serum ascorbic acid was estimated spectrophotometrically, by using 2,4-dinitrophenyl hydrazine10. The underlying principle was based on the oxidation of ascorbic acid to dehydroascorbic acid followed by coupling with 2,4- dinitrophenyl hydrazine under controlled conditions, in the presence of Thiourea as a mild reducing agent which gives red coloured osazones. The data thus obtained was subjected to statistical analysis using the PRIMER OF BIOSTATISTICS software. The comparison of variables was done using the Student paired ‘t’ test and correlation between variables was estimated by using Karl Pearson’s correlation coefficient. The significance level was considered at p Results The mean serum MDA and serum ascorbic acid levels during the follicular and luteal phases of menstrual cycle are presented as in Table 1. In the present study, the mean serum MDA (nmol/ml) level was greater during the luteal phase (4.294+1.60 nmol/ml) as compared to follicular phase (2.119+0.83 nmol/ml). The difference between MDA levels in the luteal phase was highly significant (p Lower values of serum ascorbic acid were found in luteal phase when compared to follicular phase. The mean values observed were 0.9399+0.40 mg/dl 0.8963+0.37 mg/dl in follicular phase and luteal phase, respectively. But, the changes were not significant statistically (p>0.05). In this study, a negative correlation was also observed between serum ascorbic acid (mg/dl) and serum MDA (nmol/ml) levels in both follicular and luteal phases but the correlation was found to be non-significant in both follicular phase (r value=-0.026, p>0.05) as well as in luteal phase (r value=-0.010, p>0.05). Table 1: Mean+SD levels of serum Malondialdehyde (MDA) and serum Ascorbic acid during follicular and luteal phases of menstrual cycle. Data expressed as mean and SD, * Highly significant, ** Non-significant. Discussion Present study has revealed the role of oxidative stress in the physiology of menstruation. We observed a significant higher levels of MDA while a non-significant lower levels of ascorbic acid in the luteal phase when compared with the follicular phase of the menstrual cycle. Previous studies also have correlated increased serum MDA levels during the luteal phase with the physiological phenomenon of menstruation in the healthy normal menstruating females which support our study results6,11. While, no significant differences in MDA levels throughout the normal menstrual cycle also have been reported12,13,14. Unlike our study results, a progressive significant rise in plasma ascorbic acid levels has been reported from menstrual to ovulation15. Similarly, â€Å"a sharp increase in the fasting level of plasma Vitamin C† also has been showed in some women during the middle of the menstrual cycle16. Whereas, some other researchers found no evidences of unusual variability of plasma ascorbic acid values throughout the menstrual cycle, which supports our study17. The significant rise of MDA and depletion of ascorbic acid levels during the luteal phase of menstrual cycle coincides with the increased levels of estrogen and progesterone, which is a characteristic feature of this phase18. Increased levels of estrogen and progesterone cause the proliferation of uterus while the initiation of sloughing of the endometrium is mainly due to the rise in the estrogen levels18,19,20. Thus, the high estrogen level from developing follicles may be the initiator of lipid peroxidation process21, which eventually causes the cellular injury followed by release of cytokines especially tumour necrosis factor-alpha, which generates reactive oxygen species from the tissues which in turn causes lipid peroxidation22. The generated free radicals may play an important role in spasm of the highly vascularised vessels leading to vascular necrosis and menstrual flow, when hormonal support for the endometrium is diminished. High levels of oxidative stress have been coincided with high levels of female sex hormone such as estradiol in previous studies also6,21,23. While, in other studies a significant negative correlation between these parameters in regularly menstruating females also have been established24. Whereas, some evidences show no significant correlation between ovarian hormones and oxidative stress during the follicular and luteal phases of menstrual cycle25. Present study perhaps therefore suggests that the high MDA level in the luteal phase may play an important role in the initiation of menstruation which is a well established cellular injury based phenomenon. Conclusion In conclusion, these results suggest that increase of serum MDA levels may play an important role in the physiological phenomenon of menstruation. References 1. Joseph L. Mayo. A healthy menstrual cycle. Clinical Nutrition Insights 1997; 5(9): 1-8. 2. Raharjo S, Sofos JN and Schmitt GR. Solid-phase acid extraction improves thiobarbituric acid methods to determine lipid oxidation. J Food Sci 1993; 58: 921-932. 3. Grunert RR and Phillips PH. A modification of nitroprusside method of analysis for glutathione. Arch Biochem 1951; 30(2): 217-225. 4. Sawada M and Carlson J. Superoxide radical production in plasma membrane samples from regressing corpora lutea. Can J Physiol Pharmacol 1989; 67(5): 465-471. 5. Harvey AJ, Kind KL and Thompson JG. REDOX regulation of early embryo development. Reproduction 2002; 123: 479-486. 6. Akande AA and Akinyinka AO. Serum malondialdehyde levels during menstrual cycle. Afr J Biotechnol 2005; 4(11): 1297-1299. 7. Agarwal A, Aponte-Mellado A, Premkumar BJ, Shaman A, Gupta S. The effects of oxidative stress on female reproduction: a review. Reprod Biol Endocrinol 2012; 10: 49. 8. Terada LS. Specificity in reactive oxidant signaling : Think globally, act locally. J Cell Biol 2006; 174: 615-623. 9. Satoh K. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clin Chim Acta 1978; 90(1): 37-43. 10. Natelson S. Determination of ascorbic acid by using 2,4 – dinitrophenyl hydrazine. Techniques of Clinical Chemistry. 3rd ed. Springfield, USA: Charles C. Thomas. 1971: 165-166. 11. Karowicz-Bilinska A, Plodzidym M, Krol J, Lewinska A, Bartosz G. Changes of markers of oxidative stress during menstrual cycle.Redox Rep 2008; 13(5): 237-240. 12. Serviddio G, Loverro G, Vicino M, et al. Modulation of endometrial redox balance during the menstrual cycle: relation with sex hormones. J Clin Endocrinol Metab 2002; 87(6): 2843-2848. 13. Elhadd TA, Neary R, Abdu TA, et al. Influence of the hormonal changes during the normal menstrual cycle in healthy young women on soluble adhesion molecules, plasma homocysteine, free radical markers and lipoprotein fractions. Int Angiol 2003; 22(3): 222-228. 14. Browne RW, Bloom MS, Schisterman EF, et al. Analytical and biological variation of biomarkers of oxidative stress during the menstrual cycle. Biomarkers 2008; 11: 160-183. 15. Michos C, Kiortsis DN, Evangelou A, Karkabounas S. Antioxidant protection during the menstrual cycle : The effects of estradiol on ascorbic – dehydroascorbic acid plasma levels and total antioxidant plasma status in eumenorrhoic women during the menstrual cycle. Acta Obstet Gynecol Scand 2006; 85(8): 960-965. 16. Mickelson O, Dippel AL and Todd RL. Plasma Vitamin C levels in women during the menstrual cycle. J Clin Endocrinol Metab 1943; 3(11): 600-602. 17. Hauck HM. Plasma levels and urinary excretion of ascorbic acid in women during the menstrual cycle. J Nutr 1947; 33(5): 511-514. 18. Ganong. William F. Gonads: Development and function of the reproductive system. In: Review of Medical Physiology. 13th ed. USA: Prentice-Hall Int. Inc. 1987: 364-369. 19. Llewellyn-Jones D. Everywomen A gynaecological guide for life. 4th ed. The Chancer press. Great Britain: 1986: p-48. 20. Whitley RJ, Wayne M and Nelson BW. Endocrinology. In: Teitz Textbook of Clinical Chemistry. Carl Burtis and Edward Ashwood (editors). Philadephia: Saundera company. 1992: p-1879. 21. Schisterman EF, Gaskins AJ, Mumford SL, et al. Influence of Endogenous Reproductive Hormones on F2-Isoprostane Levels in Premenopausal Women. Am J Epidemiol 2010; 172(4): 430-439. 22. Romero FJ, Bosch-Morell F, Romero MJ, Jareno EJ, Romero B, Roma MN. Lipid peroxidation products and antioxidants in human diseases. Environ Health Perspect 1998; 106: 1229-1234. 23. Sowers M, McConnell D, Jannausch ML, et al. Oestrogen metabolites in relation to isoprostanes as a measure of oxidative stress. Clin Endocrinol (Oxf) 2008; 68(5): 806-813. 24. Bednarek–Tupikowska G, Bohdanowicz–Pawlak A, Bidzinska B, Milewicz A, Antonowicz–Juchniewicz J, Andrzejak R. Serum lipid peroxide and superoxide dismutase activity in premenopausal and postmenopausal women. Gynecol Endocrinol 2001; 15(4): 298-303. 25. Lutoslawska G, Tkaczyk J, panczenco-– kresowska B, Hubner – Wozniak E, Skierska E, Gajewski AK. Plasma TBARS, blood GSH concentrations, and erythrocyte antioxidant enzyme activities in regularly menstruating women with ovulatory and anovulatory menstrual cycles. Clin Chim Acta 2003; 331(1-2): 159-163.

Overview Program Evaluation

Overview of Program Evaluation HCS/549 April 15, 2013 Overview of Program Evaluation Program evaluation is a useful resource to evaluate programs that are currently an organization or an industry is making good use of. Evaluating programs not only bring out the strengths and weaknesses but also identifies the picture of the plans implemented. The purpose of this paper is to examine the purpose for program evaluation and why it can be useful to an organization.This paper will speak of the five most common types of program evaluation models and will select one program evaluation model to describe from week one’s reading. Purpose for program evaluation â€Å"There is only one overall purpose for program evaluation activities: contributing to the provision of quality services to people in need. Program evaluation contributes to quality services by providing feedback from program activities and outcomes to those who can make changes in programs† (Posavac & Carey, 2007, p. 14 ).If there is no feedback, then the programs will be very hard to carry out and be effective. The processes of evaluation require feedback systems; similarly, feedback on behavior in organizations is also crucial for the success of an organization. Delayed feedback, not clearly associated with the examination of behavior, is not very informative. In the readings, the writers have argued that environmental problems are hard to solve because of the long delay between environmentally destructive activities and feedback indicating a weakening of natural systems. Assessing needs, measuring the implementation of programs to meet those needs, evaluating the achievement of carefully formed goals and objectives, and comparing the level of outcome with the costs involved relative to similar programs serve to provide information from which to develop program improvements† (Posavac & Carey, 2007, p. 14). This is useful for organizations because it gives them an idea of their programs and what needs help if there are any issues related to the programs evaluated. Five most common modelsA number of different approaches to evaluation have been put forward to guide the planning and implementation of program evaluations. Each of the models discussed includes emphases that are valid aspects of program evaluation. â€Å"One value of thinking about models lies in developing an appreciation of the range of questions evaluators can consider. The specific questions being addressed by an evaluation or the specific aspects of the program setting often make one or another of the models especially useful† (Posavac & Carey, 2007, p. 23-25).The models: The traditional model, Social Science model, Industrial Inspection model, Black Box Evaluation, and Objectives-Based Evaluation model, are very common and helpful in different types of organizations and environment. The model that is very interesting to use and describe is the Objective-Based Evaluation model. Objectives-Based E valuation In an effort to design an evaluation for a specific program, some approaches emphasize working with clearly stated program goals and objectives so that the degree to which such goals and objectives are achieved can be measured. This has been the most prevalent model used for program evaluation. Examining goals and objectives seems to be an essential aspect of evaluation; it is important to judge a program relative to its particular structure and what it is designed to achieve† (Posavac & Carey, 2007, p. 23-25). It is very important for organizations to clearly and closely examine their goals and objectives to successfully plan for future. At the same time, taking objectives-based evaluation to evaluate the situation is also important to know the points of view of the staff in the organization. ConclusionIn conclusion, evaluation of different types of programs is important in organizations because it bring out the ups and downs of the organization. Purpose of program evaluation is to provide feedback that can be helpful for organizations. Without feedback, the organizations programs cannot be effective or put in proper use. Also feedback on behaviors within the program evaluations is crucial for the success of an organization. The five models: The traditional model, Social Science model, Industrial Inspection model, Black Box Evaluation, and Objectives-Based Evaluation model give a ood idea of what it is like to use these models in different scenarios. The Objective-based model is the most prevalent model to use because it closely examines goals and objective that play an important part in an organization. Using this model a company or an organization can prepare for plans and goals, which can be achieved successfully. Reference Posavac, E. J. , & Carey, R. G. (2007). Program Evaluation: Methods and Case Studies (7th ed. ). Upper Saddle River, NJ: Pearson Prentice Hall.

The Blue Sword CHAPTER TEN

Harry had trouble falling asleep that night; she listened to the gentle sound the water made walking down the three stone steps, and often she stretched out her hand to touch the hilt of the blue sword that lay beside her, carefully laid upon a small carpet of blue and green and gold that she had found in a corner of a hall on her way back to her mosaic palace after the feast. She had appropriated it, rolled it up, tucked it under her arm, and glared at the woman of the household who was conducting her. The woman dropped her eyes, but did not seem unduly disturbed. Who would grudge a damalur-sol a little rug? Harimad-sol thought airily. But each time she touched the blue sword it was as if a shock ran through her, and she listened to the quiet night, hearing the echoes of sounds that had rung themselves to silence hundreds of years ago. Her restlessness made Narknon grumble at her, although the cat did not offer to leave the bed and sleep elsewhere. At last Harry tucked her hands firmly beneath her chin and fell asleep, and in her sleep she saw Aerin-sol again, and Aerin smiled at her. â€Å"Gonturan will do well for you, I think, child, as she did well for me. You can feel it in the way she hangs in your hand, can you not?† Harry, in her dream, nodded. â€Å"Gonturan is far older than I am, you know; she was given me with the weight of her own years and legend already upon her. I never knew all she might lead her bearer into – and as it was, I learned more than enough. â€Å"Gonturan has her own sense of honor, child. But she is not human, and you must not trust her as human; remember it. She is a true friend, but a friend with thoughts of her own, and the thoughts of others are dangerous.† Aerin paused, and the dream began to fade; her face was pale, and half imagined, like a cloud on a summer's dawn, with her hair the sunrise. â€Å"What luck I had, may it go with you.† Harry woke up, and found the sword gleaming blue in a light that seemed to come from the blue mosaic walls, from the blue stone in the hilt, even from the silver water of the stream. Several days passed, while some of the Riders went forth on errands; but the newest Rider did not. She spent long hours in the mosaic palace, staring at the air, which hung, or so it seemed to her, like tapestry around her; and in that tapestry was woven all of history – her own, her Homeland's, as well as Damar's. Sometimes she saw a little bright shimmer like someone tossing back a fire-red mane of hair; and sometimes she saw the glint of a blue jewel – but that was no doubt only some chance reflection from the glossy walls around her. But most of all, she slept. Mathin had been right about the sorgunal. For several days she was content to sleep, and waken to do nothing in particular, and sleep again. Narknon enjoyed it as much as she did. â€Å"I'm sure Mathin did not put any of that stuff in the porridge,† Harry said to the cat; â€Å"there's no excuse for you.† On the fourth morning Mathin came to her, and found her pacing from fountain to fountain and from wall to wall. â€Å"This is not a cage to enclose you, Hari,† he said. She turned, startled, for she had been deep in her thoughts and had not heard his approach. She smiled. â€Å"I have not felt caged. I have †¦ slept a great deal, as you warned me. It is only today I have begun to †¦ think again.† Mathin smiled in return. â€Å"Is it so ill, this thinking?† â€Å"Why am I a Rider?† she replied. â€Å"There is no reason for Corlath to make an Outlander girl, even the laprun minta, a Rider. Riders are his best. Why?† Mathin's smile twisted. â€Å"I told you, long ago – long ago, more than a week since. It is a good thing for us to have a damalur-sol. It is a good thing for us to have something to look to, for hope. Perhaps you do yourself too little honor.† Harry snorted. â€Å"Has a laprun ever been made a Rider before?† Mathin took a long time to answer. â€Å"No. You are the first to bear that burden.† â€Å"And an Outlander at that.† â€Å"You Outlanders are human, for all of that – as the Northerners are not. It is not impossible that some Outlander might have †¦ a Gift, kelar, like ours, as you do – for you do. There is something in you we recognize, and we know it is there, for Lady Aerin has chosen you herself. Corlath makes you a Rider to †¦ to take advantage of whatever it is you carry in your Outlander blood that has made you Damarian, even against your will.† Harry slowly shook her head. â€Å"Not against my will. At least not any more. But I do not understand.† â€Å"No; nor do I. Nor even does Corlath. He – † Mathin stopped. Harry looked sharply at him. â€Å"Corlath what?† The faint smile drifted across Mathin's face again. â€Å"Corlath did not steal you of his own free will. His kelar demanded it.† Harry grinned. â€Å"Yes; I had guessed, and once he told me – something of the sort. I saw dismay on his face often enough, those early days.† Mathin's face was expressionless when she raised her eyes again to his. â€Å"You have not seen dismay there for a long day since.† â€Å"No,† she agreed, and her eyes went involuntarily to the mosaic walls around her. Mathin said, â€Å"You are a token, a charm, to us, Daughter of the Riders and Rider and Damalur-sol.† â€Å"A mascot, you mean,† Harry said, but without bitterness; and still she looked at the mosaic walls. She asked timidly, not certain of her own motives, â€Å"Does Corlath have no family? I see here, in the castle, the people of the household, and the – us – Riders, but no one else. Is it only that they are cloistered – or that I am?† Mathin shook his head. â€Å"You see all there is to see. In Aerin's day the king's family filled this place; some had to live in the City, or chose to, for privacy. But kings in the latter days †¦ Corlath's father married late, and Corlath is his queen's only surviving child, for she was a frail lady. Corlath himself has not married.† Mathin smiled bleakly. â€Å"Kings should marry young and get heirs early, that their people may have one thing less to worry about. There has been no one in generations whose kelar is as strong as Corlath's; it is why the scattered folk along our borders and in the secret hearts of our Hills, who have acknowledged no Damarian king for many years, rally now to Corlath. Even where he does not go himself his messengers are alight with it.† After Mathin left her, Harry thought of taking another nap, but decided against it. Instead she rode out on Sungold, Narknon deigning to accompany them. She found at the back of the stone castle and beyond the stone stables a practice ground, stepped into the sides of the Hill, for those wishing to practice horsemanship and war. It was deserted, as though the menace of the Northerners was too near to permit of practice. But she jogged slowly around the empty field, Sungold stepping up or down as they came to each edge, and decided to practice anyway: she who was laprun victor, who had never held a sword till a few weeks ago, who was suddenly a Rider: she felt, a little wildly, that she needed all the practice she could get. She was wearing Gonturan, a little self-consciously, but she had felt somehow that it would be impolite to leave her behind. She unsheathed her and wondered if the ancient sword had ever been used to hack at straw figures and charge at dangling wooden tiles. She galloped Tsornin over poles laid on the ground, piles of stone and wooden logs, and up and down turfed banks, and over ditches. She felt a little silly; but Tsornin made it plain that he enjoyed it all, whatever it was and however humble, and Gonturan always struck true. Harry took Tsornin back to his stable and put him away with her own hands, studiously ignoring the brown-clad groom who hovered near her. Hers was the first human face she had seen since she rode out. The stables were on the same scale as the castle: large and grand, the loose-boxes the size of small fields. There were over a hundred stalls – Harry lost count when she tried to multiply them out in her head – in the barn Sungold was quartered in, and two other barns as big stood on either side of it. Sungold's stable was nearly full; sleek curious noses were thrust out at them as they left and returned. Harry saw no other men or women of the horse; they must reappear at some point, she thought, to tend the horses. Unless Hill horses can be trained to take care of themselves – it wouldn't surprise me. The silence was uncanny. Tsornin's hoofs had echoed around the practice field; and when she thanked the brown woman and said no, she needed nothing, her voice sounded strange in her ears. Over the next few days she rode out again and again, and spent some hours slaying straw men with the Dragon-Killer's sword, and then some hours riding out from the stone ring of the castle, and into the stone City, down the smooth roads. She saw mostly women and young children, but even of them there were rarely more than a few. The women watched her timidly, and smiled eagerly if she smiled at them first; and the children wanted to pet Sungold, which he was good enough to permit, and Narknon, who usually eluded them; and sometimes they brought her flowers. But the City was as empty as the castle was; there were people, but far fewer than its walls might hold. Some of this, she knew, was because the army was massing elsewhere – on the laprun fields, before the City; messengers came and went swiftly, and the gathering of forces hung heavily in the air. But most of it was because, as the king's family had dwindled, so had the king's people; there were few Damarians left. She thought again of the mounting strangenesses of her recent life; and she wished, if she was to be given to Damar, as apparently she was, that she would be given no more long pauses of inaction in which to brood about it all. One of the young women who had assisted her at her bath brought her food, in the blue front room with the fountain, or outside in the sunshine where the other fountain played; and she managed to convince her and the other women sent to wait upon her that, at least as long as there were no more banquets requiring special preparations, she might bathe herself. For three more days she slept and watched the shimmering of the air and rode Tsornin and played with Narknon. There was a friendship between the horse and the hunting-cat now, and they would chase one another around the obstacles of the practice field, Narknon's tail lashing and Sungold with his ears back in mock fury. Once the big cat had hidden behind one of the grassy banks, where Harry and Sungold could not see her; and as they rode by she leaped out at them, sailing clean over Sungold and Harry on his back. Harry ducked and Sungold swerved; and Narknon circled and came back to them with her ears back and her whiskers trembli ng in what was obviously a cat laugh. And Harry polished Gonturan and tried not to brood, and looked often at the small white scar in the palm of her hand. But with all her inevitable musings she found that a certain peace had come to her and made its way into her heart. It was not like anything she had known before, and it was only on that third day that she found a name for it: fate. Yet she wished that the business of war were not so all-consuming, that she might have someone to talk to. On the fourth day when the woman came with her afternoon meal, Corlath came with her; and evidently he was expected, although not by Harry, for there were two goblets and two plates on the tray, and far more food than she could eat alone. She was sitting on the flagstones beside the fountain in the sunshine, watching the prisms that the falling drops threw into the air; and Narknon was washing Harry's face with her razored tongue, and Harry was trying not to mind. She was trying not to mind with such concentration that she did not realize till she looked up, still dazzled by tiny intricate colors, that he was there; and she remained sitting, blinking up at him, as the woman set down her tray and retired. â€Å"May I eat with you?† he said, and Harry thought that he seemed ill at ease. â€Å"Of course,† she said. â€Å"I would – er – be honored.† She pushed Narknon's head away and started to scramble to her feet, but Corlath dropped silently down beside her, so she settled back again, grateful that her bones decided not to creak. He gave her a plate and took his own; and then sat staring into the fountain much as she had done, and she wondered, watching him, if he felt any of the queer peacefulness that crept into her with the same looking; and if he would call it by the name she had discovered. â€Å"Eight days,† she said, and his eyes drew back from the water spray and met hers. â€Å"Eight days,† she repeated. â€Å"You said less than a fortnight.† â€Å"Yes,† he replied. â€Å"We are counting the hours now.† He made a swift sweeping motion with his right hand, and Harry said suddenly: â€Å"Show me your hand.† Corlath looked puzzled for a moment, but then he held his right hand out, palm up. There was one short straight pale mark across it, obviously new; and many small white scars; she didn't have to count them to know there would be eighteen of them, the still-fresh – and longest – cut a nineteenth. She studied the hand a moment, cupping it in her own, not thinking that she was poring over a king's hand; then she looked at her own right palm. One tiny straight line looked back at her. He closed his hand and rested it on his knee. â€Å"They don't fade,† Harry said. â€Å"The old ones don't disappear.† â€Å"No,† said Corlath. â€Å"It is the yellow salve, before we make the cut; it is made of an herb called korim – forever.† She studied her own palm again for a moment. The scar cut through the lines a fortune-teller would call her life line and her heart line; and she wondered what Damarian fortune-tellers might see in her hand. She looked up at Corlath, who absently put a piece of bread in his mouth and began to chew; he was staring into the fountain again. He swallowed and said: â€Å"There is a story of one of my grandfather's Riders: the Northern border was restless then – but only restless, and this man had gone North to see what he might learn. But they caught him, and recognized him as from Damar; but he knew they would find him a little before they did, and he slashed his hand that they might not find the mark and hold him for ransom – or torture; for the Northerners, if they wish, can torture with a fine prying magic that no mind can resist.† Harry thought: If the Northerners know about the Riders' mark, they must be a bit slow not to wonder about a spy caught with a cut-up hand. Corlath continued after a moment: â€Å"He had traveled dressed as a merchant, so when he knew they would find him he freed his horse and sent it home, and took off his boots, and began to climb the near-perpendicular face of one of the Hills that is the boundary between our land and theirs. When they found him he was half mad with sunstroke and his hands and feet were as tattered as autumn leaves. They decided they had not caught a prize at all, and after they had beaten him a bit, they let him go. He finished climbing the mountain with his hands and feet, because he remembered that much of what he was doing; and just over the summit, just inside the border of Damar, his horse was waiting for him, and she took him home. He recovered from the sunstroke, but he never held a sword again.† Harry swallowed a lump of bread that didn't want to go down, and there was silence for a bit. â€Å"What happened to the mare?† she said at last. â€Å"Your Tsornin's dam is a daughter of his mare's line,† Corlath said, but it was as if he were tracing some thought of his own. â€Å"The mare lived till she was almost thirty, and dropped a foal every year till the last. Many of our best riding-horses are descended from her.† Corlath looked at her, coming back from wherever he had been. â€Å"That mare's line is called Nalan – faithful. You can see it in Tsornin's pedigree.† Harry asked lightly: â€Å"And is there a name for the line of the kings of Damar?† Corlath said, â€Å"My father's name, and his father's, and mine, is Gulkonoth: stone.† Harry looked at his right hand resting quietly on his knee. He paused and added as if inconsequentially, â€Å"There are other names for the king. One of them is Tudorsond. Scarred hand.† â€Å"Does the korim scar the foreheads of the household, and the faces of the hunt and the horse as well?† And Corlath said, â€Å"Yes.† There was a silence again, and Harry wondered how many other questions she might be able to gain answers for. She said, â€Å"Once in the mountains before the trials, Mathin said to me that he could teach me three ways of starting a fire, but that you knew a fourth. He would not tell me what the fourth was.† Corlath laughed. â€Å"I will show you one day, if you wish. Not today. Today it would give you a headache.† Harry shook her head angrily, her feeling of contentment gone. â€Å"I am tired of having things only half explained. Either I am damalur-sol, when it is convenient, or I am to be quiet and sit in a corner and behave till it is time to bring me out and show me to the troops again. Did you choose Mathin to teach me because he is close-mouthed?† Corlath looked a little abashed, and Harry guiltily remembered how much Mathin had told her, although – she defended herself – it was not enough. Never enough. But she could not help remembering his answer when she had asked him why he had been chosen for her training. â€Å"I chose Mathin because I thought he would teach you best; there are none better than he, and he is patient and tireless.† And kind, thought Harry, but she would not interrupt when she might learn something. â€Å"We of the Hills – I suppose we are all, as you say, close-mouthed; but do you think you have learned so little of us?† And Corlath looked at her – wistfully. â€Å"No,† she said, ashamed of herself. There was a pause, and she said, â€Å"Could you perhaps, please, tell me why Mathin would not tell me any of the legends about the Lady Aerin? They are a part of your lives that all of you share – and it is her sword you have given me – and the legends, why, there are a few sung even at the spring Fairs in the west, where Outlanders can hear them.† Corlath tapped his fingers, one-two-three, one-two-three, on the brim of the fountain. â€Å"Aerin is a part of your destiny, Harimad-sol. It is considered unlucky to †¦ meddle with destiny. Mathin would feel that he was doing you a disservice, speaking much of Aerin to you, and I – I find, now, that I feel the same.† Tap-tap-tap. â€Å"If you had grown up †¦ here, you would have heard them. But you did not. And if you had, perhaps you would not now be what you are. â€Å"I am sorry.† He turned and looked at her. â€Å"If – after we have met the Northerners, and the gods have decided between us, if you and I are left alive, I will tell you all the stories I know of Aerin Dragon-Killer.† He tried to smile. â€Å"I even can sing a few.† â€Å"Thank you.† Corlath's smile became more successful. â€Å"There are a very great many of them – you may not wish to hear them all.† â€Å"I do wish to hear them all,† said Harry firmly. Corlath took his hand away from the stone brim and began to shred a chunk of bread into fragments on his plate. â€Å"As for the first question,† he said, â€Å"watch.† He blinked a few times, closed his eyes, and a shudder ran through him; then he opened his eyes again and gave a hot yellow glare to the little heap of bread crumbs, which burst into flame, crackled wildly for a few minutes, and subsided into black ash. â€Å"Oh,† said Harry. Corlath looked up; his eyes were brown. They stared at one another. Harry found herself saying hastily, in a voice that was a little too high-pitched, â€Å"What is this place – here – ?† and she jerked her eyes away, and waved to the mosaic walls. â€Å"I have seen nothing else like it anywhere in the City.† Corlath shook his head. â€Å"Nor will you.† He got slowly to his feet, and looked around, and cupped his scarred hand under the fountain, and drank from it. â€Å"My father built it for my mother just after he married her. She was fond of the color blue – and I think he wanted to tell her that he did not mind that she would never carry the Blue Sword, the greatest treasure of his family, the woman's sword.† He looked down at her inscrutably, but his eyes did not focus on her. Then he turned and left her, going through the door into the castle. Two days later the army rode away from the City. Corlath and his Riders rode together down the highway from the castle to the gates of the City, with men and women of the household and the hunt and horse, and pack horses behind them; and the people of the City lined the streets and silently watched them go, although many raised their hands to their foreheads and flicked the fingers as they rode by. Harry had not seen so many before; some were refugees from northern Damarian villages, and farmers from the green lands before the Bledfi Gap. And they rode down to the plain where the army Harry had not seen, for she had not left the City since she rode into it, lay before them; and behind her she heard a sound no Damarian had heard in generations: the City's stone gates closing, heavily, mournfully. Tsornin was restless. Now, with the ranks upon ranks of the Hill army drawn up upon it, the plain looked like some other place than the plain where Harry and Tsornin had fought with blunt staves and sword points. Tsornin was too well bred to do more than fidget slightly in place; but his shoulder, when she ran her hand down it, was warmer than the morning air deserved. The muscles under the golden skin were hard; she felt that if she rapped her knuckles against his shoulder ridge it would ring like iron. She stood, a little awkwardly, in the group of Riders, only a little way into the plain from the end of the City highway. They were on a little rise of land, so they looked out and down over the rest of the company, and Harry felt unnecessarily conspicuous. â€Å"Why couldn't you be liver chestnut or something?† she whispered to Tsornin, who bowed his golden head. A new helm fitted closely down over her bound-up hair, and there were new boots on her legs, with tops that rolled up and lashed into place for battle; and she felt Gonturan hanging expectantly at her knee. Ten days were not enough to accustom herself to being a Rider, however hard she had driven herself and Tsornin round the lonely practice fields with their stiff wooden silhouettes of enemy swordsmen; and while the Riders themselves – particularly one or two: Mathin, and the merry (for a Rider) young Innath – closed ranks around her and accepted her as one of them, she could not believe that they did not themselves wonder, a little, about her presence among them. Sungold blew impatiently and began to dig a hole with one front foot. She booted his elbow with her toe and he stopped, but after a moment he lowered his head and blew again, harder, and she could feel him shifting his weight, considering if she might let him dig just a small hole. She looked around: the other horses were showing signs of stress as well. Mathin stood next to her; Windrider, although rock still, unlike the younger Tsornin, wore a dark sheen of sweat down her flank. Corlath's Fireheart was standing on his hind legs again; the king could bring him down as he chose, but Harry rather thought the horse was expressing the mood of both of them. Narknon, so far as Harry could see, was the only one of their company who remained undisturbed. She sat in front of Sungold, just beyond the reach of pawing forefeet, and washed her chest and combed her whiskers. They marched west. They crossed the low but steep ridge of mountains between the City and the desert plain that stretched far away, up to the back door of the Outlander Residency in Istan. They retraced Harry and Mathin's route, going in single endless file through the narrow paths; and they came to the desert edge at the end of the second day. Beyond the ridge they turned north. All the spies – those still living, for the North had caught a few – that Corlath had sent out in the last several years had come back in the last few months, in a rush, all with the same word: the waiting was over, the Northerners were moving. The last man of them had returned not six days before; it had taken him so long because they knew about him, and he had dodged and fled and scrambled to get away from their creeping tracking magic. His tale was that their army was only days behind him, and that it was many thousands strong. He had delayed and delayed to take a fairer tally of the total; and yet, he said, even as the army marched south, hundreds and more hundreds appeared as if out of the air to march with it. Out of the air, Harry thought, and wondered if the phrase was more than just a manner of speaking. She had been included in the council of Riders that heard the man's tale; and the candlelight seemed to cast more shadows when he was through. Yet there was no thing to be done; the army that would stand for Damar was already gathered; the plans to face the Northerners were already laid. Of the Northerners' dread captain no spy was sure; no Damarian dared get that close, for the uncanny way he was said to smell foreign blood. There were hundreds of mounted men and women now following Corlath's word; and as they rode with the eastern Hills at their right hand, they looked a great many. A few hundreds more would join as the southern army made its way to the wide plain before the Gap. But that was all. Innath, riding at her elbow, said conversationally, â€Å"Less than half of the Northern army will be mounted; and not many of them will be riding horses; and very few of their horses will match the poorest of ours. One can double our tally at least, just for our horses; for they are Damarians and will fight for Damar as fiercely as we human beings, for all that we are the only ones who talk about it.† â€Å"Yes,† said Harry, her voice only a little muffled. Noontimes they stopped briefly, loosening girths to let the horses breathe, and eating bread and dry meat and water. At night they camped behind ridges of shale and scrub, and lit fires enough to boil the terrible dry meat to a slightly more edible consistency, and rolled up in their blankets to sleep where they sat. A few of the hunting-cats and a dozen dogs were with them; but they could not spare the time at present to use them. Narknon continued at Harry's heels and, as she had done once before, began hunting on her own, and brought back some of her grisly victories to lay at Harry's pillow. As the days passed and Mathin's stew pot became generally known as the only one reliably containing fresh meat, it grew very popular. The nights were clear and quiet, and the weather-casters among them promised no sudden windstorms; the edges of the Damarian Hills were known for their unpredictable weather, where mountain storms bottled up by the steep slopes might suddenly find their way to the flatter lands where they could rage and riot as they chose. Corlath was not trying to strike at once for the center of the northern mountains and the Bledfi Gap. After the Hill army crossed the narrow range behind which the City lay, they worked their way around the curve of the mountains, trotting through the sandy sour grass and broken rock at their feet. At first this made them ride almost due north, then in an increasing arc to the west; and the sun moved across the sky before them. Often in the mornings when the mist was still lying around them, trailing from the mountains' shoulders into their camp, a little group of riders, or even a solitary figure on horseback, would loom up at them from nowhere; but Corlath always seemed to be expecting them, and they always knew what to say to the guards that they might pass; and in this way the army a little swelled its ranks. Occasionally Harry heard a woman's voice among the strangers, and this made her glad; and often she'd rub a finger over the blue gem in the hilt of Gonturan and think of the sword no man could carry. Mathin said to her once: â€Å"We did not think to see so many women – few have fought with us within any man's memory, although in Aerin's day it was different. But I think many fathers are letting their daughters join us who had not thought to till they heard of Harimad-sol, and that Gonturan went to war again.† Many of these women she met; particularly after Mathin had spoken to her, for then she began to feel a little uneasily responsible for them. Senay she saw several times – and saw too that she was wearing a sewn-together sash as if she were proud of it. Harimad-sol asked the names of the women when she had a chance, and they answered gravely; and they often gave her the back-of-hand-to-forehead gesture of respect, and none ever asked her her name, even when she was not carrying Gonturan and ought to look – she thought – like any other disheveled soldier. Most of those who came thus late to join Corlath's army did not carry a sword, and wore no sash; these were men and women who had spent their lives in their own villages, on their own farms and in their own shops, and had never attended laprun trials, nor felt the lack that they had not. One evening they rode into a hollow where nearly a hundred strangers, all mounted, and with several pack horses and hunting-beasts besides, waited for them; and Corlath rode forward with a great hearty cry of welcome, a sound nearer happiness than any Harry had heard from him since they began their march north. A rider at the head of the group rode to meet him, and they seized each other by the shoulders while their horses bumped uneasily together and rolled their eyes at each other. A third man then detached himself from the new group and joined Corlath and his friend. â€Å"Murfoth and his son, Terim,† said Mathin in Harry's ear. â€Å"Murfoth was one of the old king's friends, though he's not much more than ten years older than our king. He might have been a Rider, had he wished, but he chose instead to stay at home and look after his lands; and a good job he's made of it too. Some of our best horses now come from him, and grain to feed many more.† â€Å"We Riders,† said Innath from her other side, â€Å"as you may have noticed, tend to be fourth sons or otherwise penniless – or incurable wanderers like Mathin here – but Murfoth now, when he comes to ride with his king, can bring eighty men with him.† Innath's voice, for all its careless pride, sounded almost wistful. Harry found herself remembering her father's words to her – it seemed decades ago: â€Å"You haven't a penny, you know.† Terim was Harry's age, and when he and his father came to sit at the king's fireside he came to her and sank down beside her, folding up his long legs as all the Hillmen did. She looked at Terim and he looked at her; his look was eager and a little, to her embarrassment, reverent. â€Å"I was First at my laprun trials three years ago,† he said; â€Å"but when I took my turn against Corlath my sash was on the ground before I had a good grip on my sword.† He thumped the hilt of his sword, which jangled as it bit into the ground. â€Å"My father gave me Teksun here anyway, he said no one ever got a grip on a sword against Corlath. You did, though.† His eyes shone in the firelight. Harry ran a meditative finger over the careful seam in her sash, which she had put in under Mathin's promised tutelage. â€Å"I didn't know it was he – I never thought. And he allowed me to cross swords with him; and when I realized how much of it was allowing, I got †¦ mad.† She paused. â€Å"I was surprised too.† She frowned, remembering the awful headache she'd had for most of that day, and then the more awful sick lurch that seemed to start behind her eyes, where the headache was, and quiver all the way through her body, when she saw the face behind the scarf she had just removed. No one had called her baga for the cut at the corner of Corlath's mouth, though. She met the boy's eyes somewhat ruefully and said, â€Å"It wasn't as pleasant an experience as you might think.† Terim gave a little snort of laughter and said, â€Å"Yes, I believe you,† and Harry looked across to where Corlath sat with Terim's father and found him watching her. She wondered if he had heard what she had just said.

Success Is A Product Of Productivity - 961 Words

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